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Western blot(SDS PAGE) analysis of extracts from Jurkat cells .Using RhoA + RhoCRabbit mAb [P7A1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RHOC,ARH9,ARHC,RHOC_HUMAN,Rho-related GTP-binding protein RhoC
UniProtID :P08134,P61586
MASS(da) :21,768
MW(kDa) :22 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human RhoA + RhoC
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells..
Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Cleavage furrow.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.