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Western blot(SDS PAGE) analysis of extracts from T47D cells .Using HAI-1Rabbit mAb [2QO5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SPINT1,HAI1,UNQ223/PRO256,SPIT1_HUMAN,Kunitz-type protease inhibitor 1
UniProtID :O43278
MASS(da) :58,398
MW(kDa) :66 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human HAI-1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : T47D cells
Function : Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14).
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.