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NFIB / NF1B2 + NFIC/CTF Rabbit mAb [YM38]Cat NO.: A35382

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using NFIB / NF1B2 + NFIC/CTFRabbit mAb [YM38] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NFIB,NFIB_HUMAN,Nuclear factor 1 B-type

UniProtID :O00712,P08651

MASS(da) :47,442

MW(kDa) :45-65 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human NFIB / NF1B2 + NFIC/CTF

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : Transcriptional activator of GFAP, essential for proper brain development (PubMed:30388402). Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.