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CDC7 Rabbit mAb [0069]Cat NO.: A50562

Western blot(SDS PAGE) analysis of extracts from MCF7 CELLS .Using CDC7Rabbit mAb [0069] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CDC7,CDC7L1,CDC7_HUMAN,Cell division cycle 7-related protein kinase

UniProtID :O00311

MASS(da) :63,888

MW(kDa) :64 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CDC7

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :MCF7 CELLS 

Function : Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylate MCM2 and MCM3..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.