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Western blot(SDS PAGE) analysis of extracts from K562 cells.Using BTFRabbit mAb [QAXO] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BCLAF1,BTF,KIAA0164,BCLF1_HUMAN,Bcl-2-associated transcription factor 1
UniProtID :Q9NYF8
MASS(da) :106,122
MW(kDa) :150 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human BTF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cells
Function : Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA..
Tissue specificity :Ubiquitous.
Subcellular locationi :Cytoplasm. Nucleus. Nucleus speckle. Nucleus, nucleoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.