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Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using Coronin 1a/TACORabbit mAb [3EHW] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CORO1A,CORO1,COR1A_HUMAN,Coronin-1A
UniProtID :P31146
MASS(da) :51,026
MW(kDa) :51,57 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Coronin 1a/TACO
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes..
Tissue specificity :Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Cytoplasmic vesicle, phagosome membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.