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Western blot(SDS PAGE) analysis of extracts from OVCAR-3 .Using MesothelinRabbit mAb [432Y] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MSLN,MPF,MSLN_HUMAN,Mesothelin [Cleaved into: Megakaryocyte-potentiating factor ,Mesothelin, cleaved form]
UniProtID :Q13421
MASS(da) :68,986
MW(kDa) :40,69 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Mesothelin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :OVCAR-3
Function : Membrane-anchored forms may play a role in cellular adhesion., Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Tissue specificity :Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level)..
Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor. Golgi apparatus.,[Megakaryocyte-potentiating factor]: Secreted.,[Isoform 3]: Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.