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Rho A + B + C Rabbit mAb [0Q8Q]Cat NO.: A21827

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Rho A + B + CRabbit mAb [0Q8Q] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :RHOC,ARH9,ARHC,RHOC_HUMAN,Rho-related GTP-binding protein RhoC

UniProtID :P08134,P61586,P62745

MASS(da) :22,123

MW(kDa) :22 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Rho A + B + C

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells..

Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Cleavage furrow.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.