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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using CTGF Rabbit mAb [SV11] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CCN2,CTGF,HCS24,IGFBP8,CCN2_HUMAN,CCN family member 2
UniProtID :P29279
MASS(da) :38,091
MW(kDa) :35 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human CTGF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis..
Tissue specificity :Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested..
Subcellular locationi :Secreted, extracellular space, extracellular matrix. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.