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Western blot(SDS PAGE) analysis of extracts from NCCIT cells.Using CHD4Rabbit mAb [TR0A] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CHD4,CHD4_HUMAN,Chromodomain-helicase-DNA-binding protein 4
UniProtID :Q14839
MASS(da) :218,005
MW(kDa) :280 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CHD4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NCCIT cells
Function : Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones..
Subcellular locationi :Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.