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Western blot(SDS PAGE) analysis of extracts from Human fetal heart.Using CLIC1Rabbit mAb [H1F4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CLIC1,G6,NCC27,CLIC1_HUMAN,Chloride intracellular channel protein 1
UniProtID :O00299
MASS(da) :26,923
MW(kDa) :27 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CLIC1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal heart
Function : Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle..
Tissue specificity :Expression is prominent in heart, placenta, liver, kidney and pancreas..
Subcellular locationi :Nucleus. Nucleus membrane,Single-pass membrane protein. Cytoplasm. Cell membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.