Junctional Adhesion Molecule 1/JAM-A Rabbit mAb [6IX3]Cat NO.: A52368

Protein names ：F11R,JAM1,JCAM,UNQ264/PRO301,JAM1_HUMAN,Junctional adhesion molecule A

UniProtID ：Q9Y624

MASS(da) ：32,583

MW(kDa) ：35 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Junctional Adhesion Molecule 1/JAM-A

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：bEnd.3

Function ： Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840).., (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.., (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa..

Tissue specificity ：Expressed in endothelium, epithelium and leukocytes (at protein level)..

Subcellular locationi ：Cell junction, tight junction. Cell membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.