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Western blot(SDS PAGE) analysis of extracts from Ramos cells.Using AVENRabbit mAb [BOTR] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AVEN,AVEN_HUMAN,Cell death regulator Aven
UniProtID :Q9NQS1
MASS(da) :38,506
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human AVEN
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Ramos cells
Function : Protects against apoptosis mediated by Apaf-1.
Tissue specificity :Highly expressed in testis, ovary, thymus, prostate, spleen, small intestine, colon, heart, skeletal muscle, liver, kidney and pancreas.
Subcellular locationi :Endomembrane system,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.