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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells .Using KIF1ARabbit mAb [K5S9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KIF1A,ATSV,C2orf20,KIF1A_HUMAN,Kinesin-like protein KIF1A
UniProtID :Q12756
MASS(da) :191,064
MW(kDa) :191 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human KIF1A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Motor for anterograde axonal transport of synaptic vesicle precursors. Also required for neuronal dense core vesicles (DCVs) transport to the dendritic spines and axons. The interaction calcium-dependent with CALM1 increases vesicle motility and interaction with the scaffolding proteins PPFIA2 and TANC2 recruits DCVs to synaptic sites..
Tissue specificity :Expressed in neurons..
Subcellular locationi :Cytoplasm, cytoskeleton. Cell projection, neuron projection. Cell projection, axon. Cytoplasm, perinuclear region. Cell junction, synapse. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.