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DCTN3 Rabbit mAb [27E8]Cat NO.: A28902

Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cells .Using DCTN3Rabbit mAb [27E8] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :DCTN3,DCTN22,DCTN3_HUMAN,Dynactin subunit 3

UniProtID :O75935

MASS(da) :21,119

MW(kDa) :21 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human DCTN3

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :SH-SY5Y cells 

Function : Together with dynein may be involved in spindle assembly and cytokinesis..

Tissue specificity :Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain..

Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.