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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cells .Using DCTN3Rabbit mAb [27E8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DCTN3,DCTN22,DCTN3_HUMAN,Dynactin subunit 3
UniProtID :O75935
MASS(da) :21,119
MW(kDa) :21 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human DCTN3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cells
Function : Together with dynein may be involved in spindle assembly and cytokinesis..
Tissue specificity :Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain..
Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.