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Western blot(SDS PAGE) analysis of extracts from HepG2 cells .Using alpha 1 FetoproteinRabbit mAb [P298] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AFP,HPAFP,FETA_HUMAN,Alpha-fetoprotein
UniProtID :P02771
MASS(da) :68,678
MW(kDa) :70 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human alpha 1 Fetoprotein
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Tissue specificity :Plasma. Synthesized by the fetal liver and yolk sac.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.