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alpha 1 Fetoprotein Rabbit mAb [P298]Cat NO.: A40336

Western blot(SDS PAGE) analysis of extracts from HepG2 cells .Using alpha 1 FetoproteinRabbit mAb [P298] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :AFP,HPAFP,FETA_HUMAN,Alpha-fetoprotein

UniProtID :P02771

MASS(da) :68,678

MW(kDa) :70 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human alpha 1 Fetoprotein

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells 

Function : Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Tissue specificity :Plasma. Synthesized by the fetal liver and yolk sac.

Subcellular locationi :Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.