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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using NDUFAF5Rabbit mAb [9TQ5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NDUFAF5,C20orf7,NDUF5_HUMAN,Arginine-hydroxylase NDUFAF5, mitochondrial
UniProtID :Q5TEU4
MASS(da) :38,918
MW(kDa) :38 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human NDUFAF5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Arginine hydroxylase involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages (PubMed:18940309, PubMed:27226634). Acts by mediating hydroxylation of 'Arg-111' of NDUFS7 (PubMed:27226634). May also have methyltransferase activity (Probable)..
Subcellular locationi :Mitochondrion inner membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.