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Western blot(SDS PAGE) analysis of extracts from MCF7 CELLS .Using HIKESHIRabbit mAb [CA34] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HIKESHI,C11orf73,HSPC138,HSPC179,HSPC248,HIKES_HUMAN,Protein Hikeshi
UniProtID :Q53FT3
MASS(da) :21,628
MW(kDa) :22 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human HIKESHI
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 CELLS
Function : Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus..
Subcellular locationi :Cytoplasm. Cytoplasm, cytosol. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.