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Western blot(SDS PAGE) analysis of extracts from MCF7 CELLS .Using SEC22BRabbit mAb [Y24L] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SEC22B,SEC22L1,SC22B_HUMAN,Vesicle-trafficking protein SEC22b
UniProtID :O75396
MASS(da) :24,741
MW(kDa) :28 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SEC22B
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 CELLS
Function : SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER..
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type IV membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus, cis-Golgi network membrane. Golgi apparatus, trans-Golgi network membrane. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.