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Western blot(SDS PAGE) analysis of extracts from Recombinant human Entactin/NID protein .Using Entactin/NIDRabbit mAb [0ISA] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NID1,NID,NID1_HUMAN,Nidogen-1
UniProtID :P14543
MASS(da) :136,377
MW(kDa) :130 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Entactin/NID
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Recombinant human Entactin/NID protein
Function : Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Subcellular locationi :Secreted, extracellular space, extracellular matrix, basement membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.