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Entactin/NID Rabbit mAb [0ISA]Cat NO.: A59561

Western blot(SDS PAGE) analysis of extracts from Recombinant human Entactin/NID protein .Using Entactin/NIDRabbit mAb [0ISA] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NID1,NID,NID1_HUMAN,Nidogen-1

UniProtID :P14543

MASS(da) :136,377

MW(kDa) :130 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Entactin/NID

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Recombinant human Entactin/NID protein 

Function : Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Subcellular locationi :Secreted, extracellular space, extracellular matrix, basement membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.