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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using TWEAKR/FN14Rabbit mAb [CCXG] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TNFRSF12A,FN14,TNR12_HUMAN,Tumor necrosis factor receptor superfamily member 12A
UniProtID :Q9NP84
MASS(da) :13,911
MW(kDa) :14-17 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TWEAKR/FN14
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins..
Tissue specificity :Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.