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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using MGST1Rabbit mAb [XTAU] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MGST1,GST12,MGST,MGST1_HUMAN,Microsomal glutathione S-transferase 1
UniProtID :P10620
MASS(da) :17,599
MW(kDa) :18 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MGST1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.
Tissue specificity :Highly expressed in liver.
Subcellular locationi :Microsome. Mitochondrion outer membrane,Peripheral membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.