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Calmegin Rabbit mAb [ATDQ]Cat NO.: A12572

Western blot(SDS PAGE) analysis of extracts from Human testis .Using CalmeginRabbit mAb [ATDQ] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CLGN,CLGN_HUMAN,Calmegin

UniProtID :O14967

MASS(da) :70,039

MW(kDa) :100 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Calmegin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Human testis 

Function : Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity)..

Tissue specificity :Detected in testis (at protein level). Detected in testis..

Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.