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Western blot(SDS PAGE) analysis of extracts from Human testis .Using CalmeginRabbit mAb [ATDQ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CLGN,CLGN_HUMAN,Calmegin
UniProtID :O14967
MASS(da) :70,039
MW(kDa) :100 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Calmegin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human testis
Function : Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity)..
Tissue specificity :Detected in testis (at protein level). Detected in testis..
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.