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Western blot(SDS PAGE) analysis of extracts from NIH3T3 cells.Using IL-12ARabbit mAb [NRS1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IL12A,NKSF1,IL12A_HUMAN,Interleukin-12 subunit alpha
UniProtID :P29459
MASS(da) :24,874
MW(kDa) :35 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human IL-12A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH3T3 cells
Function : Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.