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Western blot(SDS PAGE) analysis of extracts from human fetal kidney.Using OsteopontinRabbit mAb [Y8K4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SPP1,BNSP,OPN,PSEC0156,OSTP_HUMAN,Osteopontin
UniProtID :P10451
MASS(da) :35,423
MW(kDa) :60-65 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Osteopontin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human fetal kidney
Function : Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.., Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity..
Tissue specificity :Bone. Found in plasma.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.