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Osteopontin Rabbit mAb [Y8K4]Cat NO.: A15152

Western blot(SDS PAGE) analysis of extracts from human fetal kidney.Using OsteopontinRabbit mAb [Y8K4] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SPP1,BNSP,OPN,PSEC0156,OSTP_HUMAN,Osteopontin

UniProtID :P10451

MASS(da) :35,423

MW(kDa) :60-65 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Osteopontin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human fetal kidney

Function : Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.., Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity..

Tissue specificity :Bone. Found in plasma.

Subcellular locationi :Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.