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Western blot(SDS PAGE) analysis of extracts from Mouse lung.Using Claudin 5Rabbit mAb [RO48] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CLDN5,AWAL,TMVCF,CLD5_HUMAN,Claudin-5
UniProtID :O00501
MASS(da) :23,147
MW(kDa) :20 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Claudin 5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse lung
Function : Plays a major role in tight junction-specific obliteration of the intercellular space..
Subcellular locationi :Cell junction, tight junction. Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.