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CD97 Rabbit mAb [4877]Cat NO.: A24272

Western blot(SDS PAGE) analysis of extracts from tonsil.Using CD97Rabbit mAb [4877] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ADGRE5,CD97,AGRE5_HUMAN,Adhesion G protein-coupled receptor E5 [Cleaved into: Adhesion G protein-coupled receptor E5 subunit alpha,Adhesion G protein-coupled receptor E5 subunit beta]

UniProtID :P48960

MASS(da) :91,869

MW(kDa) :80 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CD97

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :tonsil

Function : Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration..

Tissue specificity :Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompanied by detectable levels of soluble CD97 in the synovial fluid.

Subcellular locationi :Cell membrane,Multi-pass membrane protein.,[Adhesion G protein-coupled receptor E5 subunit alpha]: Secreted, extracellular space.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.