Myosin Phosphatase + Myosin Phosphatase 2 Rabbit mAb [EJ17]Cat NO.: A15083

Protein names ：PPP1R12A,MBS,MYPT1,MYPT1_HUMAN,Protein phosphatase 1 regulatory subunit 12A

UniProtID ：O14974

MASS(da) ：115,281

MW(kDa) ：110 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Myosin Phosphatase + Myosin Phosphatase 2

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells

Function ： Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity..

Tissue specificity ：Expressed in striated muscles, specifically in type 2a fibers (at protein level)..

Subcellular locationi ：Cytoplasm. Cytoplasm, cytoskeleton, stress fiber.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.