ATP6V1D Rabbit mAb [270F]Cat NO.: A71116

Protein names ：ATP6V1D,ATP6M,VATD,VATD_HUMAN,V-type proton ATPase subunit D

UniProtID ：Q9Y5K8

MASS(da) ：28,263

MW(kDa) ：28 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ATP6V1D

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：293T cells 

Function ： Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (PubMed:21844891)..

Subcellular locationi ：Membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle membrane,Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, cilium.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.