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Western blot(SDS PAGE) analysis of extracts from HACAT.Using Cytokeratin 16/K16Rabbit mAb [51O4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KRT16,KRT16A,K1C16_HUMAN,Keratin, type I cytoskeletal 16
UniProtID :P08779
MASS(da) :51,268
MW(kDa) :51 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cytokeratin 16/K16
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HACAT
Function : Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance..
Tissue specificity :Expressed in the corneal epithelium (at protein level)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.