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Cytokeratin 16/K16 Rabbit mAb [51O4]Cat NO.: A53169

Western blot(SDS PAGE) analysis of extracts from HACAT.Using Cytokeratin 16/K16Rabbit mAb [51O4] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :KRT16,KRT16A,K1C16_HUMAN,Keratin, type I cytoskeletal 16

UniProtID :P08779

MASS(da) :51,268

MW(kDa) :51 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cytokeratin 16/K16

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HACAT

Function : Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance..

Tissue specificity :Expressed in the corneal epithelium (at protein level)..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.