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FANCI Rabbit mAb [7K1C]Cat NO.: A66734

Western blot(SDS PAGE) analysis of extracts from HeLa cells treated with 500ng/ml Mitomycin C for 6h.Using FANCIRabbit mAb [7K1C] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :FANCI,KIAA1794,FANCI_HUMAN,Fanconi anemia group I protein

UniProtID :Q9NVI1

MASS(da) :149,324

MW(kDa) :149 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human FANCI

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells treated with 500ng/ml Mitomycin C for 6h

Function : Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage..

Subcellular locationi :Nucleus. Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.