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Western blot(SDS PAGE) analysis of extracts from human kidney carcinoma .Using SCFRabbit mAb [38ZV] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KITLG,MGF,SCF,SCF_HUMAN,Kit ligand [Cleaved into: Soluble KIT ligand ]
UniProtID :P21583
MASS(da) :30,899
MW(kDa) :18 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SCF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human kidney carcinoma
Function : Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Subcellular locationi :[Isoform 1]: Cell membrane,Single-pass type I membrane protein.,[Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane,Single-pass type I membrane protein. Cell projection, lamellipodium. Cell projection, filopodium.,[Soluble KIT ligand]: Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.