TGF alpha Rabbit mAb [0W26]Cat NO.: A42959

Protein names ：TGFA,TGFA_HUMAN,Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha ]

UniProtID ：P01135

MASS(da) ：17,006

MW(kDa) ：15 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TGF alpha

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：human fetal skin

Function ： TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Tissue specificity ：Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines..

Subcellular locationi ：[Transforming growth factor alpha]: Secreted, extracellular space.,[Protransforming growth factor alpha]: Cell membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.