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Western blot(SDS PAGE) analysis of extracts from MeWo.Using TRP2/DCTRabbit mAb [HLAP] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DCT,TYRP2,TYRP2_HUMAN,L-dopachrome tautomerase
UniProtID :P40126
MASS(da) :59,145
MW(kDa) :69,85 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TRP2/DCT
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MeWo
Function : Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA)..
Subcellular locationi :Melanosome membrane,Single-pass type I membrane protein. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.