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Western blot(SDS PAGE) analysis of extracts from HT1080 CELLS.Using C10orf46/CAC1Rabbit mAb [37R6] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CACUL1,C10orf46,CAC1,CACL1_HUMAN,CDK2-associated and cullin domain-containing protein 1
UniProtID :Q86Y37
MASS(da) :41,064
MW(kDa) :37 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human C10orf46/CAC1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT1080 CELLS
Function : Cell cycle associated protein capable of promoting cell proliferation through the activation of CDK2 at the G1/S phase transition..
Tissue specificity :Ubiquitously expressed with highest expression in the mammary gland and large intestine. Highly expressed in cancer tissues and cancer cell lines. During cell cycle progression expression is high in G1/S, low in the middle of S phase, and increases again in S/G2 phase..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.