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Western blot(SDS PAGE) analysis of extracts from HFF-1 cells .Using Collagen IRabbit mAb [P559] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COL1A2,CO1A2_HUMAN,Collagen alpha-2 chain
UniProtID :P08123
MASS(da) :129,314
MW(kDa) :220,35 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Collagen I
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HFF-1 cells
Function : Type I collagen is a member of group I collagen (fibrillar forming collagen).
Tissue specificity :Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.