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Western blot(SDS PAGE) analysis of extracts from Neuro-2a cells.Using GNAO1Rabbit mAb [K9E4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GNAO1,GNAO_HUMAN,Guanine nucleotide-binding protein G subunit alpha
UniProtID :P09471
MASS(da) :40,051
MW(kDa) :37 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GNAO1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Neuro-2a cells
Function : Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14.
Subcellular locationi :Cell membrane. Membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.