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Western blot(SDS PAGE) analysis of extracts from Raw 264.7 cells.Using KCTD5Rabbit mAb [8C25] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KCTD5,KCTD5_HUMAN,BTB/POZ domain-containing protein KCTD5
UniProtID :Q9NXV2
MASS(da) :26,093
MW(kDa) :26 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human KCTD5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : Raw 264.7 cells
Function : Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex (PubMed:18573101). Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4 (PubMed:19361449)..
Subcellular locationi :Cytoplasm, cytosol. Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.