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Western blot(SDS PAGE) analysis of extracts from U937 cells.Using D4 GDIRabbit mAb [23X6] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ARHGDIB,GDIA2,GDID4,RAP1GN1,GDIR2_HUMAN,Rho GDP-dissociation inhibitor 2
UniProtID :P52566
MASS(da) :22,988
MW(kDa) :27 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human D4 GDI
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U937 cells
Function : Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133)..
Tissue specificity :Detected in bone marrow, thymus and spleen..
Subcellular locationi :Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.