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Western blot(SDS PAGE) analysis of extracts from SW480 cells .Using InvolucrinRabbit mAb [O414] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IVL,INVO_HUMAN,Involucrin
UniProtID :P07476
MASS(da) :68,479
MW(kDa) :120 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Involucrin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SW480 cells
Function : Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Tissue specificity :Keratinocytes of epidermis and other stratified squamous epithelia.
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.