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Western blot(SDS PAGE) analysis of extracts from Y79 cells .Using Otx1 + Otx2Rabbit mAb [688P] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :OTX1,OTX1_HUMAN,Homeobox protein OTX1
UniProtID :P32242,P32243
MASS(da) :37,327
MW(kDa) :75 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Otx1 + Otx2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Y79 cells
Function : Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Tissue specificity :Expressed in brain. Detected in the anterior part of the neural fetal retina (at protein level)..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.