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Western blot(SDS PAGE) analysis of extracts from recombinant protein.Using Flt3 ligand/Flt3LRabbit mAb [HF06] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FLT3LG,FLT3L_HUMAN,Fms-related tyrosine kinase 3 ligand
UniProtID :P49771
MASS(da) :26,416
MW(kDa) :26 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Flt3 ligand/Flt3L
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :recombinant protein
Function : Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Subcellular locationi :[Isoform 1]: Cell membrane,Single-pass type I membrane protein.,[Isoform 2]: Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.