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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells .Using CEACAM5Rabbit mAb [YV04] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CEACAM5,CEA,CEAM5_HUMAN,Carcinoembryonic antigen-related cell adhesion molecule 5
UniProtID :P06731
MASS(da) :76,796
MW(kDa) :180-200 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CEACAM5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression,induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).., (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer..
Tissue specificity :Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon..
Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.