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Western blot(SDS PAGE) analysis of extracts from Jurkat cells .Using IL-16Rabbit mAb [4BK5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IL16,IL16_HUMAN,Pro-interleukin-16 [Cleaved into: Interleukin-16 ]
UniProtID :Q14005
MASS(da) :141,752
MW(kDa) :75-40,80 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human IL-16
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4., [Isoform 1]: May act as a scaffolding protein that anchors ion channels in the membrane., Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
Tissue specificity :[Isoform 3]: Expressed in hemopoietic tissues, such as resting T-cells, but undetectable during active T-cell proliferation.
Subcellular locationi :[Interleukin-16]: Secreted.,[Isoform 1]: Cytoplasm.,[Isoform 3]: Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.