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Western blot(SDS PAGE) analysis of extracts from A431 cells.Using P cadherinRabbit mAb [79BS] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDH3,CDHP,CADH3_HUMAN,Cadherin-3
UniProtID :P22223
MASS(da) :91,418
MW(kDa) :120 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human P cadherin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells
Function : Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells,cadherins may thus contribute to the sorting of heterogeneous cell types.
Tissue specificity :Expressed in some normal epithelial tissues and in some carcinoma cell lines..
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.