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DEPTOR/DEPDC6 Rabbit mAb [3E27]Cat NO.: A91508

Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using DEPTOR/DEPDC6 Rabbit mAb [3E27] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :DEPTOR,DEPDC6,DPTOR_HUMAN,DEP domain-containing mTOR-interacting protein

UniProtID :Q8TB45

MASS(da) :46,294

MW(kDa) :48 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human DEPTOR/DEPDC6

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :MCF-7 cells

Function : Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.