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Western blot(SDS PAGE) analysis of extracts from HaCaT .Using Cytokeratin 6Rabbit mAb [IQL9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KRT6C,KRT6E,K2C6C_HUMAN,Keratin, type II cytoskeletal 6C
UniProtID :P48668
MASS(da) :60,025
MW(kDa) :56 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cytokeratin 6
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HaCaT
Tissue specificity :Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.