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Eg5 Rabbit mAb [T76B]Cat NO.: A45818

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Eg5Rabbit mAb [T76B] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :KIF11,EG5,KNSL1,TRIP5,KIF11_HUMAN,Kinesin-like protein KIF11

UniProtID :P52732

MASS(da) :119,159

MW(kDa) :130 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Eg5

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Motor protein required for establishing a bipolar spindle during mitosis (PubMed:19001501). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769)..

Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton, spindle pole.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.