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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Eg5Rabbit mAb [T76B] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KIF11,EG5,KNSL1,TRIP5,KIF11_HUMAN,Kinesin-like protein KIF11
UniProtID :P52732
MASS(da) :119,159
MW(kDa) :130 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Eg5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Motor protein required for establishing a bipolar spindle during mitosis (PubMed:19001501). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769)..
Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton, spindle pole.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.