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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using GRASP65Rabbit mAb [8ELH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GORASP1,GOLPH5,GRASP65,GORS1_HUMAN,Golgi reassembly-stacking protein 1
UniProtID :Q9BQQ3
MASS(da) :46,482
MW(kDa) :46 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GRASP65
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (PubMed:26363069). Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (PubMed:16489344). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed:21884936)..
Subcellular locationi :Golgi apparatus, cis-Golgi network membrane,Peripheral membrane protein,Cytoplasmic side. Endoplasmic reticulum-Golgi intermediate compartment membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.