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Western blot(SDS PAGE) analysis of extracts from human melanoma.Using MelanARabbit mAb [3L8L] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MLANA,MART1,MAR1_HUMAN,Melanoma antigen recognized by T-cells 1
UniProtID :Q16655
MASS(da) :13,157
MW(kDa) :20 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MelanA
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human melanoma
Function : Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes..
Tissue specificity :Expression is restricted to melanoma and melanocyte cell lines and retina.
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.