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MelanA Rabbit mAb [3L8L]Cat NO.: A64234

Western blot(SDS PAGE) analysis of extracts from human melanoma.Using MelanARabbit mAb [3L8L] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MLANA,MART1,MAR1_HUMAN,Melanoma antigen recognized by T-cells 1

UniProtID :Q16655

MASS(da) :13,157

MW(kDa) :20 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MelanA

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human melanoma

Function : Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes..

Tissue specificity :Expression is restricted to melanoma and melanocyte cell lines and retina.

Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.